Eastóscadh DNA géanómach ó whea(modh coirnín maighnéadach）

Tá cruithneacht ar cheann de na barraí móra bia sa tSín, mar sin tá taighde ar a bhitheolaíocht mhóilíneach an-tábhachtach freisin agus tá an teicneolaíocht ag éirí níos forbartha. Whether it is gene cloning and transformation, genetic map construction, braite géinitíopa sonrach, molecular marker-assisted breeding or DNA fingerprint database construction, they are widely applied in wheat breeding. High-quality DNA extraction is the basis for systematic analysis and research on plants at the molecular level. There are many methods for extracting plant genomic DNA, but traditional methods use harmful reagents such as phenol and chloroform, and involve many steps and take a long time. Dá bhrí sin, it is recommended to use the simple, gasta, non-toxic and environmentally friendly magnetic bead method for extracting plant genomic DNA.

The plant genomic DNA extraction reagent (modh coirníní maighnéadach) de Shanghai Lingjun Biteicneolaíocht Co., Teo. is mainly used for extracting genomic DNA from various tissues of different plants. The lysis buffer directly lyses the plant samples to release nucleic acids. Under the action of the binding buffer, the magnetic beads selectively adsorb the nucleic acids in the lysis buffer. The washing buffer is used to clean the magnetic beads to remove a small amount of impurities adsorbed on them. Under the action of the elution buffer, the magnetic beads release the adsorbed nucleic acids, thus obtaining high-quality genomic DNA. The entire process is convenient to operate and is especially suitable for automated nucleic acid extraction platforms. The extracted genomic DNA has a high yield, íonachta ard, cáilíocht cobhsaí agus iontaofa, agus is féidir iad a chur i bhfeidhm ar thurgnaimh éagsúla bitheolaíochta mhóilíneacha iartheachtacha.

The product advantages of our company’s plant genomic DNA extraction reagent (modh coirníní maighnéadach) are as follows:

1. It can achieve excellent extraction results for both fresh and dried samples.

2. The extracted products have high purity, toradh ard, agus bannaí electrophoretic iomlána.

3. It has strong universality and is suitable for extracting genomic DNA from samples such as leaves, torthaí, and seeds of different types of plants.

4. The usage process is safe and does not require liquid nitrogen grinding. The product does not contain harmful reagents such as phenol and chloroform.

5. This kit can be used for manual extraction or can be well adapted for various high-throughput extraction instruments.

6. It is fast and simple. The entire extraction process can yield high-purity genomes within 20-60 nóiméad.

We used wheat leaves as the experimental material and extracted the wheat genomic DNA using the plant genomic DNA extraction reagent (modh coirníní maighnéadach) provided by Shanghai Lingjun Biotechnology Co., Teo. The reagent kit included lysis binding solution, coirníní maighnéadacha, PK, DTT, washing solution, and elution solution, among other reagents.

 Is iad seo a leanas na torthaí turgnamhacha:

Uimhir	Sampla	Méid Samplála	A260/A280	A260/A230	Conc.(de/ul)	Toradh (μg)
1	wheat leaf	20mg	1.881	2.223	372.3	37.2
2	wheat leaf	20mg	1.879	2.216	375.35	37.5
3	wheat leaf	20mg	1.871	2.212	371	37.1
4	wheat leaf	20mg	1.878	2.21	370.95	37.1

Torthaí leictreafóiréis:

M:Marcóir （2K，1K，750bp，500bp，250bp，100bp）;

1-4：wheat leaf（20mg）

Anailís Thorthaí:

The test results of the ultra-micro nucleic acid analyzer show:

① The value of A260/A280 is above 1.8, and the value of A260/A230 is above 2.0, indicating that the purity of the extracted products is high.

② The nucleic acid extraction yield of 20mg wheat leaf samples is above 37 µg.

The results of agarose gel electrophoresis show:

① Wheat leaves all have bright bands and good integrity.

Mar fhocal scoir: Using this kit to extract the genomic DNA of wheat leaf samples, the purity, yield and electrophoretic bands of the extracted products are all high.

Soláthraí

Shanghai Lingjun Biteicneolaíocht Co., Teo.bunaíodh i 2016 atá ina mhonaróir gairmiúil d'ábhair bithmhaighnéadacha agus imoibrithe eastóscadh aigéid núicléacha.

Tá taithí shaibhir againn in eastóscadh agus íonú aigéad núicléasach, íonú próitéine, scaradh cille, ceimileacanacht, agus réimsí teicniúla eile.

Úsáidtear ár gcuid táirgí go forleathan i go leor réimsí, mar thástáil leighis, tástáil ghéiniteach, taighde ollscoile, pórú géiniteach, agus mar sin de. Ní chuirimid ar fáil ach táirgí ach freisin is féidir tabhairt faoi OEM, ODM, agus riachtanais eile. Má tá riachtanas gaolmhar agat, bíodh leisce ort teagmháil a dhéanamh linn .