밀에서 게놈 DNA 추출(자기 비드 방식)

밀은 중국의 주요 식량작물 중 하나이다., 그래서 분자생물학에 대한 연구도 매우 중요하고 기술도 점점 발전하고 있습니다.. Whether it is gene cloning and transformation, genetic map construction, specific genotype detection, molecular marker-assisted breeding or DNA fingerprint database construction, they are widely applied in wheat breeding. High-quality DNA extraction is the basis for systematic analysis and research on plants at the molecular level. There are many methods for extracting plant genomic DNA, but traditional methods use harmful reagents such as phenol and chloroform, and involve many steps and take a long time. 그러므로, it is recommended to use the simple, 빠른, non-toxic and environmentally friendly magnetic bead method for extracting plant genomic DNA.

The plant genomic DNA extraction reagent (자기비드 방식) ~의 상하이 링쥔 생명공학 유한회사, 주식회사. is mainly used for extracting genomic DNA from various tissues of different plants. The lysis buffer directly lyses the plant samples to release nucleic acids. Under the action of the binding buffer, the magnetic beads selectively adsorb the nucleic acids in the lysis buffer. The washing buffer is used to clean the magnetic beads to remove a small amount of impurities adsorbed on them. Under the action of the elution buffer, the magnetic beads release the adsorbed nucleic acids, thus obtaining high-quality genomic DNA. The entire process is convenient to operate and is especially suitable for automated nucleic acid extraction platforms. The extracted genomic DNA has a high yield, 고순도, 안정적이고 믿을 수 있는 품질, and can be applied to various downstream molecular biology experiments.

The product advantages of our company’s plant genomic DNA extraction reagent (자기비드 방식) are as follows:

1. It can achieve excellent extraction results for both fresh and dried samples.

2. The extracted products have high purity, 높은 수율, and complete electrophoretic bands.

3. It has strong universality and is suitable for extracting genomic DNA from samples such as leaves, 과일, and seeds of different types of plants.

4. The usage process is safe and does not require liquid nitrogen grinding. 본 제품에는 페놀, 클로로포름 등 유해한 시약이 포함되어 있지 않습니다..

5. This kit can be used for manual extraction or can be well adapted for various high-throughput extraction instruments.

6. It is fast and simple. The entire extraction process can yield high-purity genomes within 20-60 분.

We used wheat leaves as the experimental material and extracted the wheat genomic DNA using the plant genomic DNA extraction reagent (자기비드 방식) provided by Shanghai Lingjun Biotechnology Co., 주식회사. The reagent kit included lysis binding solution, 자기 구슬, PK, DTT, washing solution, and elution solution, among other reagents.

 The experimental results are as follows:

숫자	견본	Sampling Amount	A260/A280	A260/A230	농도.(/ul의)	수율(μg)
1	wheat leaf	20mg	1.881	2.223	372.3	37.2
2	wheat leaf	20mg	1.879	2.216	375.35	37.5
3	wheat leaf	20mg	1.871	2.212	371	37.1
4	wheat leaf	20mg	1.878	2.21	370.95	37.1

Electrophoresis results:

중:마커（2K，1케이，750bp，500bp，250bp，100bp);

1-4：wheat leaf（20mg）

결과 분석:

The test results of the ultra-micro nucleic acid analyzer show:

① The value of A260/A280 is above 1.8, 그리고 A260/A230의 가치는 위에 있습니다 2.0, indicating that the purity of the extracted products is high.

② The nucleic acid extraction yield of 20mg wheat leaf samples is above 37 µg.

The results of agarose gel electrophoresis show:

① Wheat leaves all have bright bands and good integrity.

결론적으로: Using this kit to extract the genomic DNA of wheat leaf samples, the purity, yield and electrophoretic bands of the extracted products are all high.

공급자

상하이 링쥔 생명공학 유한회사, 주식회사에 설립되었습니다 2016 생체자성재료 및 핵산추출시약 전문제조업체입니다..

핵산 추출 및 정제 분야에서 풍부한 경험을 보유하고 있습니다., 단백질 정제, 세포 분리, 화학발광, 및 기타 기술 분야.

우리의 제품은 많은 분야에서 널리 사용됩니다., 의료 테스트와 같은, 유전자 검사, 대학 연구, 유전자 육종, 등. 우리는 제품을 제공할 뿐만 아니라 OEM도 수행할 수 있습니다., ODM, 그리고 다른 필요. 관련된 필요사항이 있는 경우, 저희에게 연락하게 자유롭게 느끼십시오 .