Extraction de l'ADN génomique du blé(méthode des billes magnétiques）

Le blé est l'une des principales cultures vivrières en Chine, la recherche sur sa biologie moléculaire est donc également très importante et la technologie est de plus en plus avancée.. Whether it is gene cloning and transformation, genetic map construction, specific genotype detection, molecular marker-assisted breeding or DNA fingerprint database construction, they are widely applied in wheat breeding. High-quality DNA extraction is the basis for systematic analysis and research on plants at the molecular level. There are many methods for extracting plant genomic DNA, but traditional methods use harmful reagents such as phenol and chloroform, and involve many steps and take a long time. Donc, it is recommended to use the simple, rapide, non-toxic and environmentally friendly magnetic bead method for extracting plant genomic DNA.

The plant genomic DNA extraction reagent (méthode des billes magnétiques) de Société de biotechnologie Shanghai Lingjun., Ltée. is mainly used for extracting genomic DNA from various tissues of different plants. The lysis buffer directly lyses the plant samples to release nucleic acids. Under the action of the binding buffer, the magnetic beads selectively adsorb the nucleic acids in the lysis buffer. The washing buffer is used to clean the magnetic beads to remove a small amount of impurities adsorbed on them. Under the action of the elution buffer, the magnetic beads release the adsorbed nucleic acids, thus obtaining high-quality genomic DNA. The entire process is convenient to operate and is especially suitable for automated nucleic acid extraction platforms. The extracted genomic DNA has a high yield, haute pureté, qualité stable et fiable, et peut être appliqué à diverses expériences de biologie moléculaire en aval.

The product advantages of our company’s plant genomic DNA extraction reagent (méthode des billes magnétiques) are as follows:

1. It can achieve excellent extraction results for both fresh and dried samples.

2. The extracted products have high purity, rendement élevé, et bandes électrophorétiques complètes.

3. It has strong universality and is suitable for extracting genomic DNA from samples such as leaves, fruits, and seeds of different types of plants.

4. The usage process is safe and does not require liquid nitrogen grinding. The product does not contain harmful reagents such as phenol and chloroform.

5. This kit can be used for manual extraction or can be well adapted for various high-throughput extraction instruments.

6. It is fast and simple. The entire extraction process can yield high-purity genomes within 20-60 minutes.

We used wheat leaves as the experimental material and extracted the wheat genomic DNA using the plant genomic DNA extraction reagent (méthode des billes magnétiques) provided by Shanghai Lingjun Biotechnology Co., Ltée. The reagent kit included lysis binding solution, perles magnétiques, PK, TNT, washing solution, and elution solution, among other reagents.

 The experimental results are as follows:

Nombre	Échantillon	Sampling Amount	A260/A280	A260/A230	Conc..(de/ul)	Rendement (μg)
1	wheat leaf	20mg	1.881	2.223	372.3	37.2
2	wheat leaf	20mg	1.879	2.216	375.35	37.5
3	wheat leaf	20mg	1.871	2.212	371	37.1
4	wheat leaf	20mg	1.878	2.21	370.95	37.1

Electrophoresis results:

M.:Marqueur (2K，1K，750pb，500pb，250pb，100pb）;

1-4：wheat leaf（20mg）

Analyse des résultats:

The test results of the ultra-micro nucleic acid analyzer show:

① The value of A260/A280 is above 1.8, et la valeur de A260/A230 est supérieure 2.0, indicating that the purity of the extracted products is high.

② The nucleic acid extraction yield of 20mg wheat leaf samples is above 37 µg.

The results of agarose gel electrophoresis show:

① Wheat leaves all have bright bands and good integrity.

En conclusion: Using this kit to extract the genomic DNA of wheat leaf samples, the purity, yield and electrophoretic bands of the extracted products are all high.

Fournisseur

Société de biotechnologie Shanghai Lingjun., Ltd.a été établi en 2016 qui est un fabricant professionnel de matériaux biomagnétiques et de réactifs d'extraction d'acide nucléique.

Nous avons une riche expérience dans l'extraction et la purification des acides nucléiques, purification des protéines, séparation cellulaire, chimiluminescence, et autres domaines techniques.

Nos produits sont largement utilisés dans de nombreux domaines, comme les tests médicaux, tests génétiques, recherche universitaire, sélection génétique, et ainsi de suite. Nous fournissons non seulement des produits, mais pouvons également entreprendre des OEM, ODM, et autres besoins. Si vous avez un besoin connexe, n'hésitez pas à nous contacter .