ウィアからのゲノム DNA の抽出(磁気ビーズ法）

小麦は中国の主要な食用作物の一つです, そのため分子生物学の研究も非常に重要であり、その技術はますます進歩しています。. Whether it is gene cloning and transformation, genetic map construction, specific genotype detection, molecular marker-assisted breeding or DNA fingerprint database construction, they are widely applied in wheat breeding. High-quality DNA extraction is the basis for systematic analysis and research on plants at the molecular level. There are many methods for extracting plant genomic DNA, but traditional methods use harmful reagents such as phenol and chloroform, and involve many steps and take a long time. したがって, it is recommended to use the simple, 急速な, non-toxic and environmentally friendly magnetic bead method for extracting plant genomic DNA.

The plant genomic DNA extraction reagent (磁気ビーズ法) の 上海霊軍生物技術有限公司, 株式会社. is mainly used for extracting genomic DNA from various tissues of different plants. The lysis buffer directly lyses the plant samples to release nucleic acids. Under the action of the binding buffer, the magnetic beads selectively adsorb the nucleic acids in the lysis buffer. The washing buffer is used to clean the magnetic beads to remove a small amount of impurities adsorbed on them. Under the action of the elution buffer, the magnetic beads release the adsorbed nucleic acids, thus obtaining high-quality genomic DNA. The entire process is convenient to operate and is especially suitable for automated nucleic acid extraction platforms. The extracted genomic DNA has a high yield, 高純度, 安定した信頼できる品質, さまざまな下流の分子生物学実験に適用できます.

The product advantages of our company’s plant genomic DNA extraction reagent (磁気ビーズ法) are as follows:

1. It can achieve excellent extraction results for both fresh and dried samples.

2. The extracted products have high purity, 高収量, 完全な電気泳動バンド.

3. It has strong universality and is suitable for extracting genomic DNA from samples such as leaves, 果物, and seeds of different types of plants.

4. The usage process is safe and does not require liquid nitrogen grinding. The product does not contain harmful reagents such as phenol and chloroform.

5. This kit can be used for manual extraction or can be well adapted for various high-throughput extraction instruments.

6. It is fast and simple. The entire extraction process can yield high-purity genomes within 20-60 分.

We used wheat leaves as the experimental material and extracted the wheat genomic DNA using the plant genomic DNA extraction reagent (磁気ビーズ法) provided by Shanghai Lingjun Biotechnology Co., 株式会社. The reagent kit included lysis binding solution, 磁気ビーズ, PK, DTT, washing solution, and elution solution, among other reagents.

 The experimental results are as follows:

番号	サンプル	Sampling Amount	A260/A280	A260/A230	濃度.(の/ul)	収量（μg）
1	wheat leaf	20mg	1.881	2.223	372.3	37.2
2	wheat leaf	20mg	1.879	2.216	375.35	37.5
3	wheat leaf	20mg	1.871	2.212	371	37.1
4	wheat leaf	20mg	1.878	2.21	370.95	37.1

Electrophoresis results:

M:マーカー（2K），1K，750血圧，500血圧，250血圧，100血圧）;

1-4：wheat leaf（20mg）

結果分析:

The test results of the ultra-micro nucleic acid analyzer show:

① The value of A260/A280 is above 1.8, A260/A230の値が上である 2.0, indicating that the purity of the extracted products is high.

② The nucleic acid extraction yield of 20mg wheat leaf samples is above 37 μg.

The results of agarose gel electrophoresis show:

① Wheat leaves all have bright bands and good integrity.

結論は: Using this kit to extract the genomic DNA of wheat leaf samples, the purity, yield and electrophoretic bands of the extracted products are all high.

サプライヤー

上海霊軍生物技術有限公司, 株式会社に設立されました 2016 生体磁性材料と核酸抽出試薬の専門メーカーです.

核酸抽出・精製の経験が豊富です。, タンパク質の精製, 細胞分離, 化学発光, その他の技術分野.

当社の製品はさまざまな分野で広く使用されています, 医療検査など, 遺伝子検査, 大学の研究, 遺伝子育種, 等々. 製品のご提供だけでなくOEMも承ります, ODM, その他のニーズ. 関連するニーズがある場合, お気軽にお問い合わせください .