Extracción de ADN genómico de trigo.(método de cuentas magnéticas）

El trigo es uno de los principales cultivos alimentarios en China, por lo que la investigación sobre su biología molecular también es muy importante y la tecnología es cada vez más avanzada. Whether it is gene cloning and transformation, genetic map construction, detección de genotipo específico, molecular marker-assisted breeding or DNA fingerprint database construction, they are widely applied in wheat breeding. High-quality DNA extraction is the basis for systematic analysis and research on plants at the molecular level. There are many methods for extracting plant genomic DNA, but traditional methods use harmful reagents such as phenol and chloroform, and involve many steps and take a long time. Por lo tanto, it is recommended to use the simple, rápido, non-toxic and environmentally friendly magnetic bead method for extracting plant genomic DNA.

The plant genomic DNA extraction reagent (método de cuentas magnéticas) de Shanghai Lingjun Biotecnología Co., Limitado. is mainly used for extracting genomic DNA from various tissues of different plants. The lysis buffer directly lyses the plant samples to release nucleic acids. Under the action of the binding buffer, the magnetic beads selectively adsorb the nucleic acids in the lysis buffer. The washing buffer is used to clean the magnetic beads to remove a small amount of impurities adsorbed on them. Bajo la acción del tampón de elución., the magnetic beads release the adsorbed nucleic acids, thus obtaining high-quality genomic DNA. The entire process is convenient to operate and is especially suitable for automated nucleic acid extraction platforms. The extracted genomic DNA has a high yield, alta pureza, calidad estable y confiable, y se puede aplicar a varios experimentos de biología molecular posteriores..

The product advantages of our company’s plant genomic DNA extraction reagent (método de cuentas magnéticas) are as follows:

1. It can achieve excellent extraction results for both fresh and dried samples.

2. The extracted products have high purity, alto rendimiento, y bandas electroforéticas completas.

3. It has strong universality and is suitable for extracting genomic DNA from samples such as leaves, frutas, and seeds of different types of plants.

4. The usage process is safe and does not require liquid nitrogen grinding. El producto no contiene reactivos nocivos como fenol y cloroformo..

5. This kit can be used for manual extraction or can be well adapted for various high-throughput extraction instruments.

6. It is fast and simple. The entire extraction process can yield high-purity genomes within 20-60 minutos.

We used wheat leaves as the experimental material and extracted the wheat genomic DNA using the plant genomic DNA extraction reagent (método de cuentas magnéticas) provided by Shanghai Lingjun Biotechnology Co., Limitado. The reagent kit included lysis binding solution, cuentas magnéticas, PK, DTT, washing solution, and elution solution, among other reagents.

 Los resultados experimentales son los siguientes.:

Número	Muestra	Cantidad de muestreo	A260/A280	A260/A230	concentrado.(de/ul)	Rendimiento (μg)
1	wheat leaf	20mg	1.881	2.223	372.3	37.2
2	wheat leaf	20mg	1.879	2.216	375.35	37.5
3	wheat leaf	20mg	1.871	2.212	371	37.1
4	wheat leaf	20mg	1.878	2.21	370.95	37.1

Resultados de electroforesis:

METRO:Marcador (2K)，1k，750pb，500pb，250pb，100pb）;

1-4：wheat leaf（20mg）

Análisis de resultados:

The test results of the ultra-micro nucleic acid analyzer show:

① The value of A260/A280 is above 1.8, y el valor de A260/A230 está por encima 2.0, indicating that the purity of the extracted products is high.

② The nucleic acid extraction yield of 20mg wheat leaf samples is above 37 µg.

The results of agarose gel electrophoresis show:

① Wheat leaves all have bright bands and good integrity.

En conclusión: Using this kit to extract the genomic DNA of wheat leaf samples, the purity, yield and electrophoretic bands of the extracted products are all high.

Proveedor

Shanghai Lingjun Biotecnología Co., Limitado.se estableció en 2016 que es un fabricante profesional de materiales biomagnéticos y reactivos de extracción de ácidos nucleicos..

Tenemos una rica experiencia en extracción y purificación de ácidos nucleicos., purificación de proteínas, separación celular, quimioluminiscencia, y otros campos técnicos.

Nuestros productos son ampliamente utilizados en muchos campos., como pruebas médicas, pruebas genéticas, investigación universitaria, mejoramiento genético, etcétera. No solo proporcionamos productos sino que también podemos realizar OEM, ODM, y otras necesidades. Si tienes una necesidad relacionada, no dude en contactarnos .