Using Silica-Based Magnetic Beads to Extract DNA/RNA from Whole Blood

1.Introduction

With the rapid development of biotechnology and the advancement of precision medicine, its market demand continues to grow. Shanghai Lingjun Biotechnology Co., Ltd.’s magnetic beads (Product No. 160014) offer unique advantages in whole blood genomic DNA extraction kits.

In fields such as molecular diagnostics, genomics research, and viral detection, the efficient and high-purity extraction of nucleic acids (DNA/RNA) from complex samples—especially whole blood—is a critical first step for the success of downstream experiments. Therefore, whole blood genomic DNA extraction has broad application prospects in multiple areas.

2. Silica-based magnetic beads

The 160014 magnetic beads, used in conjunction with Lingjun’s Whole Blood Genomic DNA Extraction Kit, are designed for the extraction, enrichment, and purification of genomic nucleic acids from fresh, frozen, or anticoagulant-treated blood samples. Under the action of guanidine salts, sample cells are lysed to release nucleic acids. The 160014 magnetic beads specifically adsorb the nucleic acids, separating them from other impurities in the sample. After washing with a buffer to remove proteins and residual guanidine salts, the purified nucleic acids are eluted and collected via magnetic adsorption. The resulting product can be used in PCR amplification, nucleic acid detection analysis, gene mutation detection, sequencing, and other experiments.

3. Our product offers the following advantages

1、For difficult-to-lyse samples, the 160014 magnetic beads combined with the extraction kit enable simultaneous lysis and binding without the need to pause and add other reagents midway.

2、The extraction process does not require the addition of Proteinase K, allowing for transportation and storage at room temperature.

3、The extracted genomic DNA exhibits high purity, high yield, and intact electrophoresis bands.

4、The extraction efficiency is high, with the entire process taking approximately 20 minutes, making it suitable for extracting nucleic acids from 200–400 μL of whole blood samples.

5、Strong compatibility, allowing it to be used with automated nucleic acid extraction equipment from various manufacturers.

4. Experimental Case

 Experimental results of extracting genomic DNA from fresh blood samples of different healthy individuals using 160014 magnetic beads:

Sample number	Sample volume（μL）	A260/A280	A260/A230	Conc.(ng/μL)	Elution volume（μL）	Nucleic acid yield（μg）
Sample 1	200	1.950	1.493	59.10	80	4.728
Sample 2		1.964	1.523	60.65		4.852
Sample 3		1.928	1.469	74.30		5.944
Sample 4		1.967	1.481	62.75		5.020
Sample 5		1.982	1.797	81.30		6.504
Sample 6		2.006	1.890	78.75		6.300
Sample 7		1.932	1.524	66.10		5.288
Sample 8		1.945	1.540	72.90		5.832
Sample 9		1.956	1.728	99.90		7.992
Sample 10		1.991	1.832	88.80		7.104
Sample 11		1.943	1.485	78.10		6.248
Sample 12		1.975	1.473	38.15		3.052
Sample 13		1.917	1.459	105.80		8.464
Sample 14		1.988	1.657	58.05		4.644
AVG		1.963	1.578	70.37		5.630

Agarose gel electrophoresis:

Analysis of Results:

1、The extraction time is approximately 20 minutes, and genomic DNA was successfully extracted from all 14 human whole blood samples with high stability.

2、The extracted products show high purity: the 260/280 ratios all reached above 1.9, and the 260/230 ratios all reached above 1.4.

3、The extracted products exhibit high yield: the yield from 200 μL samples was above 3 μg, meeting the requirements for downstream applications.

4、The electrophoresis bands are intact, with no degradation or smearing.

5. Application

High-quality nucleic acids extracted from whole blood can be used in various advanced molecular biology applications, including:

1、Genetic Testing: Such as genetic disease screening and pharmacogenomics.

2、Pathogen Detection: Quantitative detection of viruses (e.g., HBV, HCV, HIV) and bacterial load.

3、Forensics: STR analysis and DNA fingerprinting.

4、Next-Generation Sequencing (NGS): Whole genome sequencing (WGS) and targeted sequencing, which require inhibitor-free and intact input DNA.

5、Biopreservation: Long-term storage of high-quality DNA.

Supplier

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 which is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence, and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but also can undertake OEM, ODM, and other needs. If you have a related need, please feel free to contact us .