न्यूक्लिक एसिड निष्कर्षण के दौरान डीएनए की तुलना में आरएनए अधिक आसानी से क्यों नष्ट हो जाता है??

जिस कारण आर.एन.ए (राइबोन्यूक्लिक एसिड) डीएनए की तुलना में अधिक आसानी से नष्ट हो जाता है (डिऑक्सीराइबोन्यूक्लिक अम्ल) during निष्कर्षण is mainly related to their different chemical structures and improper operation during extraction.

मैं. Differences in chemical structure:

1. RNA is a single-stranded structure, which is more unstable than the double-stranded structure of DNA.

2. Hydroxyl at the 2′ position: The ribose in RNA molecules contains a hydroxyl group (-ओह) at the 2′ position, while the deoxyribose in DNA has a hydrogen atom at this position. This extra hydroxyl group makes the RNA molecule more unstable because it can promote spontaneous hydrolysis reactions, resulting in RNA chain breaks.

द्वितीय. The influence of RNAse in the extraction system:

1. Endogenous RNAse: It exists in cells and can continue to degrade RNA even after cell lysis.

2. Exogenous RNAse: It is widely present in the environment, such as air, skin, and laboratory supplies (gloves, test tubes, वगैरह।).

तृतीय. Improper experimental operation:

1. The reagents and instruments used have not been treated with DEPC (diethylpyrocarbonate) and cannot effectively inactivate RNA enzymes.

2. The inhibitor is ineffective, or the dosage is insufficient, and the activity of RNA enzymes cannot be effectively inhibited.

3. More experimental samples or higher homogenization temperatures lead to increased RNA enzyme activity.

4. Changes in pH and temperature, especially under alkaline conditions, can easily cause RNA bases to be deaminated, resulting in chain breaks; high temperatures can also cause partial or complete degradation of RNA molecules.

चतुर्थ. What should be done to reduce RNA degradation？

In order to reduce RNA degradation during extraction, a series of measures need to be taken, including using RNAse-free laboratory supplies, operating at low temperatures, using RNA stabilizers, using finished kits, वगैरह. These methods effectively reduce the risk of RNA degradation and ensure that high-quality RNA samples are obtained for downstream analysis.

आपूर्तिकर्ता परिचय

शंघाई लिंगजुन बायोटेक्नोलॉजी कंपनी, लिमिटेडमें स्थापित किया गया था 2016 जो बायोमैग्नेटिक सामग्रियों और न्यूक्लिक एसिड निष्कर्षण अभिकर्मकों का एक पेशेवर निर्माता है.

हमारे पास न्यूक्लिक एसिड निष्कर्षण और शुद्धिकरण में समृद्ध अनुभव है, प्रोटीन शुद्धि, कोशिका पृथक्करण, केमिलुमिनसेंस और अन्य तकनीकी क्षेत्र.

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