डीएनए फ़्रैगमेंट सॉर्टिंग के लिए कुशल समाधान कैसे प्रदान करें?

वर्तमान में, आणविक जीव विज्ञान अनुसंधान में कई समस्याओं के लिए आकार के अनुसार डीएनए की ग्रेडिंग की आवश्यकता होती है, जिसे खंड छँटाई के रूप में जाना जाता है. Electrophoresis on agarose gel allows the separation of DNA in the range of 10 ⁵~hundreds of base pairs. इसके विपरीत, electrophoresis on acrylamide gel allows the separation of DNA in the range of thousands of base pairs. To achieve high resolution in these separation processes, the size difference to separate two fragments should be 10% and a nucleic acid content of up to 5 को 10 milligrams.

The selective precipitation method, a combination of polyethylene glycol and biological magnetic beads, stands out as a more efficient and convenient approach to fragment sorting. In high concentrations of PEG and NaCl solutions, PEG disrupts the hydration layer outside DNA molecules, causing them to aggregate and precipitate. This exposes the negatively charged phosphate groups, which then form a ‘salt bridge’ with the carboxyl groups on the magnetic beads’ surface, allowing DNA to be adsorbed onto the beads.

डीएनए जितना लंबा होगा, अधिक नकारात्मक रूप से आवेशित फॉस्फेट समूह सतह पर उजागर होते हैं, making the entire molecule more negatively charged and easier to adsorb onto magnetic beads, and it can be recovered with lower concentrations of PEG and NaCl. The shorter the DNA, the higher the concentration of PEG and NaCl needed to break down the hydration layer on its surface thoroughly, exposing enough negatively charged phosphate groups to be adsorbed by magnetic beads and then recovered. If you want to recover shorter DNA fragments, आपको बड़ी मात्रा में चुंबकीय मोतियों को जोड़ने की आवश्यकता है. The ratio of magnetic bead suspension to the sample also affects the size of fragments screening. As the increasing of the concentration of magnetic bead suspension, the magnetic beads will increase their adsorption capacity for small fragments in addition to large fragments.

When a specific length of DNA fragment is to be separated by magnetic beads, two rounds of sorting are typically performed. In the first round, the magnetic beads bind to DNA with higher molecular weight, which is then removed by discarding the beads. The second round involves the remaining product, where the magnetic beads bind to DNA with higher molecular weight and remove DNA with lower molecular weight by discarding the supernatant.

Both double-stranded DNA, single-stranded DNA, and RNA can dissociate negatively charged phosphate groups in solution so they can be purified and recovered with magnetic beads. तथापि, single-stranded DNA and RNA cannot be screened for fragment size. The secondary structure of double-stranded DNA is generally a relatively stable linear double-helix molecule. In the presence of PEG, the number of negatively charged phosphate groups dissociated is positively correlated with its length. तथापि, RNA and single-stranded DNA have complex secondary structures, which prevent the exposed negative groups from being positively correlated with length, making it impossible to screen fragments like DNA. इसके अलावा, due to the varying stability of DNA and RNA at different pH values, the pH of the buffers used for purifying DNA and RNA are different because DNA and RNA have different stability under different pH conditions.

Shanghai Lingjun Biotechnology fragment sorting magnetic beads are independently developed and produced from raw materials to finished magnetic beads, with strict quality control to ensure that the performance and consistency of the final product meet high standards and ensure a stable supply of the product. The company continuously invests in research and development, always maintaining an absolute emphasis on product quality, customer satisfaction, and market competitiveness.

आपूर्तिकर्ता परिचय

शंघाई लिंगजुन बायोटेक्नोलॉजी कंपनी, लिमिटेडमें स्थापित किया गया था 2016 जो बायोमैग्नेटिक सामग्रियों और न्यूक्लिक एसिड निष्कर्षण अभिकर्मकों का एक पेशेवर निर्माता है.

हमारे पास न्यूक्लिक एसिड निष्कर्षण और शुद्धिकरण में समृद्ध अनुभव है, प्रोटीन शुद्धि, कोशिका पृथक्करण, केमिलुमिनसेंस और अन्य तकनीकी क्षेत्र.

हमारे उत्पाद कई क्षेत्रों में व्यापक रूप से उपयोग किए जाते हैं, जैसे चिकित्सा परीक्षण, आनुवंशिक परीक्षण, विश्वविद्यालय अनुसंधान, आनुवंशिक प्रजनन, और इसी तरह. हम न केवल उत्पाद उपलब्ध कराते हैं बल्कि ओईएम का कार्य भी कर सकते हैं, ओडीएम, और अन्य जरूरतें. यदि आपके पास संबंधित आवश्यकताएं हैं, कृपया बेझिझक हमसे संपर्क करें sales01@lingjunbio.com.